TS EAMCET Medical 8 May 2019 Shift 2 Paper

hi a restriction enzyme digestion experiment, a DNA fragment of 1 kilobases length is used. This fragment harbors three 5'-GAATTC-3’ restriction sites. .After the restriction digestion of this fragment with Eco RL 3 DNA fragments conesponding to 500.300 & 100 base pairs are observed in the agarose gel electrophoresis (AGE). Predict the positions of the restriction site (GAATTC) in the undigested 1 kb- fragment which will result in the observed bands in AGE.