TS EAMCET Medical 2018 May 3 Shift 1Paper

The larger DNA fragment which is resulted upon digestion of PBR322 using PstI and PvuII is ligated with a DNA insert and transformed to E.coli cells.
Assertion (A) : E.coli colonies harbouring recombinant vector cannot be obtained on tetracyclin-agar plates on spreading.
Reason (R) : Because, pan of the ampR gene has been deleted while cloning the insert.
Which of the following is true?