Class 11 NEET Biology Photosynthesis in Higher Plants

Chemiosmosis requires a membrane, a proton pump, a proton gradient and ATP synthase. Energy is used to pump protons across a membrane to create a gradient or a high concentration of protons within the thylakoid lumen.
The NADP reductase enzyme is located on the stroma side of the membrane. Along with the electrons that come from the acceptor of electrons of PS I, protons are necessary for reduction of NADP+to NADPH+H+
The process does not involve breaking of electron gradient.