Class 12 NEET Biology Biotechnology Principles and Processes

The correct answer is option (1), because the restriction sites for restriction endonucleases Bam HI and Sal I are present within the tet ‌R gene in the pBR 322 cloning vector.
Options (2), (3) and (4) are incorrect because the restriction sites of Pst I and PvuI are present within the ampR gene in the pBR 322 cloning vector.