KVPY SX SB Exam 20141 Question Paper

A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to produce a 3000 bp and a 2000 bp bands when visualised on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A (A1) is present at the 100${}^{\mathrm{th}}$ base, the order in which the remaining sites (A2, B1 and B2) are present is