KVPY SX SB Exam 2013 Question Paper

A scientist has cloned an 8 Kb fragment of a mouse gene into the Eco RI site o f a vector of 6 Kb size. The cloned DNA has no other Eco RI site within. Digestions of the cloned DNA is shown below. Which one of the following sets of DNA fragments generated by digestion with both Eco RI and Bam HI as shown in (iii) is from the gene?