KVPY SX SB Exam 2013 Question Paper

Question : 113 of 120

Marks: +1, -0

Eco RI and Rsa I restriction endonucleases require 6 and 4 bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes

0 Eco RI and 10 Rsa I
1 Eco RI and 29 Rsa I
4 Eco RI and 69 Rsa I
2 Eco RI and 39, Rsa I

Validate

Solution:

For EcoR

\I(1/4)^6×10000=2.44

sites present
For Rsa

\I(1/4)^4×10000=39.06

Using probability rules

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