KVPY SX SB Exam 2012 Question Paper

Question : 115 of 120

Marks: +1, -0

The restriction endonuclease EcoR-I recognises and cleaves DNA sequence as shown below
5’-GAATTC-3’
3’-CTTAAG-5’
What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence?

Validate

Solution:

Eco RI is an example of six-cutter restriction endonuclease. It usually cleaves once in every 4096 bp.

(1/4)^6=1/4096

Given length of DNA fragment= 10 Kb = 10000 bp
Hence, Probable no. of cleaving sites

=10000/4096=2.44

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