Graduate Pharmacy Aptitude Test (GPAT) 2007 Solved Paper

The principle of ELISA is based on these two observations
(P) Antibodies and antigens can attach to solid-phase supports and stillmaintain their full immunological capabilities
(Q) Antibodies complex with enzymes allowing full separation of antigen molecules
(R) Antigens and antibodies can bebonded to enzymes and resulting complexes are stillfully functional both immunologically and enzymatically
(S) Enzymatic action is crucial for converting the antigens to render them sutible for binding to antibodies